AccuStart™ Genotyping ToughMix® is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues

• Optimized buffer chemistry destabilizes single base-pair mismatch probes, providing superior allelic discrimination and improved cluster separation for SNP detection assays
• ToughMix reagent technology neutralizes a broad spectrum of PCR inhibitors
• Easy-to-use 2X concentrated SuperMix with AccuVue plate loading dye

A key component of AccuStart Genotyping ToughMix, ROX is an ultra pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. AccuStart Genotyping ToughMix, ROX is compatible with both fast and conventional PCR cycling protocols. Real-Time PCR Instrument Compatibility:
Applied Biosystems 5700
Applied Biosystems 7000
Applied Biosystems 7300
Applied Biosystems 7700
Applied Biosystems 7900
Applied Biosystems 7900HT
Applied Biosystems 7900 HT Fast
Applied Biosystems StepOne™
Applied Biosystems StepOnePlus™

Contents:
2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, AccuVue blue qPCR dye, and stabilizers

Storage conditions:
AccuStart Genotyping ToughMix, ROX is stable for 2 years when stored in a constant temperature freezer at –20°C. For convenience, it may be stored unfrozen at 2 to 8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing does not affect PCR performance.

Precautions and Disclaimer:
For research use only. Not intended for any animal or human therapeutic or diagnostic use. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. AccuStart Genotyping ToughMix, ROX contains all required reaction components, except primers, probes, and DNA template. The light blue color of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.