Corning® Nu-Serum® growth media supplement provides a low-protein alternative to newborn calf, foetal bovine, and other sera routinely used for cell culture. The low-protein content facilitates protein purification, virus production, monoclonal antibody production and screening, and increases the frequency of successful transfection of cells.

Formulation: Frozen solution containing 25% newborn serum, EGF, ECGS, insulin, human transferrin, triiodothyronine, progesterone, estradiol-17ß, testosterone, hydrocortisone, selenous acid, o-phosphorylethanolamine, glucose, amino acids, vitamins, and other trace elements and nutrients contained in the Ham's F12 medium base.

Tested at a concentration of 10% for the ability to stimulate a >= 100-fold increase in growth of BHK-21 and a >= 13-fold increase in growth of BALB/c-3T3 cells. Tested for total protein content, pH, and osmolarity (information included with product). Filtered (0.2 µm membrane) and tested and found negative for the presence of bacteria, fungi, mycoplasma, and viruses (bovine diarrhea virus, bovine parvovirus, bovine adenovirus, reovirus, and rabies virus); also tested for the absence of cytopathic effects (CPE), inclusion bodies and hemadsorption. Tested for endotoxin (LAL assay).

31:657, 1987), monoclonal antibody production and screening (Sjogren-Janssen, E., e al., J. Immunol. Meth. 84:357, 1985), and increases the frequency of successful transfection of cells (Seed, B., et al., Proc. Natl. Acad. Sci. USA 84:3365, 1987; Duval, N., et al., J. Cell Biol. 118:641, 1992.