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Specifications
- Packaging:Glass bottle
- Description:HEPES buffer solution (1 mol/l)
- Storage temperature:Ambient
- Volume:100 ml
- Supplier No.:25-060-CI
Specifications
About this item
HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2
Unlike the low pKa of bicarbonate, HEPES has a pKa of 7.66 making it a much stronger buffer in the pH 7.2-7.6 range than bicarbonate buffer. Potential disadvantages of HEPES include toxicity over 100 mM and lack of nutritive value to the cell.
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