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Specifications
- Method / Format:Column (silica-based)
- Prep size:Mini
- Refine target molecule:Total DNA
- Sample type:FFPE tissues
- Target molecule:DNA
- Description:E.Z.N.A.® FFPE DNA Kit
- Size:50 Preps
- Description:E.Z.N.A.® FFPE DNA Kit
- Cat. no.:CA11006-984
- Supplier No.:D3399-01
Specifications
About this item
The E.Z.N.A.® FFPE DNA Kit is designed for fast and easy purification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections
After adjusting the binding conditions with ethanol, the lysate is applied to the MicroElute DNA column to bind DNA. Cellular debris and proteins are effectively removed during the wash steps. High quality purified DNA is then eluted in elution buffer or water and is ready for applications such as PCR and next-generation sequencing.
Paraffin removal can be performed using a xylene and ethanol method or an efficient heat treatment. Samples are incubated in a specialized lysis buffer along with Proteinase K to reverse crosslinking, effectively releasing short and long DNA fragments.