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Specifications
- Application:qPCR dye
- Description:PerfeCTa® SYBR® Green FastMix®
- Cat. no.:CA101414-276
- No. of Reactions:5000
- Supplier No.:95072-05K
Specifications
About this item
This unique combination of proprietary buffer, stabilizers, and AccuFast™ Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, and specificity, along with a robust fluorescent signal using fast, or conventional, cycling protocols with SYBR® Green qPCR
- 2× concentrated reagents minimize pipetting steps and improve accuracy
- Ultrapure, hot start AccuStart enzyme technology and anti-foaming technology
- FastMix formulation supports both fast and standard thermal cycling conditions
AccuFast™ Taq DNA polymerase is a key component of this reaction mix. This hot-start Taq contains a unique mixture of monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step (>48 hours at room temperature). Similar to our AccuStart™ Taq DNA polymerase, these antibodies are irreversibly inactivated during the initial PCR denaturation step. However, unlike other antibody hot-start polymerases, activation of AccuFast™ Taq is instantaneous at 95°C. Rapid recovery of fully active, unmodified Taq DNA polymerase is critical for efficient extension kinetics. Replication of fragments up to 200bp is complete in less than 20 seconds at 60°C.
Kits are available for all real-time PCR instrument platforms including those requiring normalization with ROX™ reference dye. Platforms not requiring passive reference dye include Roche LightCycler® 480, Bio-Rad® iQ™, MyiQ™, iQ™5, Opticon®, and Chromo4™, Corbett Rotor-Gene®, Eppendorf® Mastercycler®, and Cepheid® SmartCycler®. Kits with Low ROX™ reference dye are for use with platforms including AB 7500 and Stratagene® Mx™. Kits with ROX™ reference dye are for use with platforms including AB 7000, 7300, 7700, 7900, and StepOne™.
Highly specific amplification is crucial to successful qPCR with SYBR® Green I dye technology because this dye binds to and detects any dsDNA generated during amplification.
Supplier: Corning
$935.45
Supplier: VWR International
$194.14 to $694.12