Plasmid DNA Isolation Kit- E.Z.N.A. Fastfilter Endo-Free Plasmid Maxiprep Kit (25 preps). Isolation of up to 1.5mg endotoxin-free Plasmid DNA in less than 30 minutes with maxi-psin column, Lysate clearance syringe included

E.Z.N.A.® Endo-Free Plasmid Maxi Kit, Omega Bio-tek®

Catalog #: CA101319-342

CAS Number:

E.Z.N.A.® Endo-Free Plasmid Maxi Kit, Omega Bio-tek®

Catalog #: CA101319-342

CAS Number:

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Specifications

Method / Format:
Column (silica-based)
Prep size:
Maxi
Refine target molecule:
Plasmid (endotoxin-free)
Sample type:
Bacteria
Target molecule:
Plasmid
Description:
Endo-Free Plasmid Maxi Kit
Size:
25 Preps
Description:
Endo-Free Plasmid Maxi Kit
Cat. no.:
CA101319-342
Includes:
Solution I, Solution II, Buffer N3, RNase A, ETR Binding Buffer, ETR Wash Buffer, Buffer HB, DNA Wash Buffer, Endo-Free Elution Buffer, HiBind® DNA Maxi Columns, 50mL Collection Tubes, 50mL Lysate Clearance Syringes
Supplier No.:
D6926-03

Specifications

About this item

The Endo-Free Plasmid Maxi Kits provide an efficient method for the large-scale isolation of endotoxin-free plasmid DNA

Endotoxin-Free
Format Maxi

The Endo-Free Plasmid Maxi Kits provide an efficient method for the large-scale isolation of endotoxin-free plasmid DNA

Following lysis, endotoxins are removed from the cleared lysate with the addition of ETR Solution. Plasmid DNA is then further purified by adsorbing to the HiBind® silica membrane. An easy wash step is followed by the elution of the bound plasmid DNA in elution buffer. Up to 200µg of high-quality plasmid DNA can be purified from 30–50mL of overnight culture (D6915), and up to 1mg from 100–200mL of overnight culture (D6926). Purified DNA samples are ready to be used in downstream applications such as automated fluorescent DNA sequencing, restriction enzyme digestion, transfection of mammalian cells, cloning, and PCR amplification.

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