To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Format:Midi
- Method / Format:Column (silica-based)
- Prep size:Midi
- Refine target molecule:Plasmid
- Sample type:Bacteria
- Target molecule:Plasmid
- Description:Plasmid Midi Kit
- Size:25 Preps
- Description:Plasmid Midi Kit
- Cat. no.:CA101318-874
- Includes:Solution I, Solution II, Solution III, Buffer HB, DNA Wash Buffer, Elution Buffer, RNase A, HiBind® DNA Midi Column I (25), 15 mL Collection Tubes (25)
- Supplier No.:D6904-03
Specifications
About this item
The E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200µg of plasmid DNA in less than 60 minutes
Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple "Bind,Wash,Elute" procedure, thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50mL of an overnight culture in LB medium will typically produce 200µg of high-copy number plasmid DNA. When working with low-copy number plasmids, the starting culture volume can be increased up to 100mL. High-quality DNA is ready for immediate use in routine molecular biology applications such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.
The E.Z.N.A.® Plasmid Midi Kit offers a novel and reliable method of isolating up to 200µg of plasmid DNA in less than 60 minutes
Plasmid purification using the E.Z.N.A.® Plasmid Midi Kit follows a simple "Bind,Wash,Elute" procedure, thereby making it possible for multiple samples to be processed. Although yields vary according to plasmid copy number, E.coli strain, and conditions of growth, 50mL of an overnight culture in LB medium will typically produce 200µg of high-copy number plasmid DNA. When working with low-copy number plasmids, the starting culture volume can be increased up to 100mL. High-quality DNA is ready for immediate use in routine molecular biology applications such as automated fluorescent sequencing, restriction enzyme digestion, and transfection screening.
This kit combines HiBind® membrane technology with the time-tested consistency of alkaline SDS lysis of bacterial cells to deliver high-quality plasmid DNA. The DNase/RNase-free HiBind® DNA Midi Columns operate by eliminating time-consuming phenol-chloroform extractions and alcohol precipitations.
Supplier: Kimberly-Clark
$34.40 to $500.24
Supplier: Thermo Fisher Scientific
$67.38 to $298.06
Supplier: BD
$46.00 to $367.00
Supplier: Spectrum Laboratories
$287.86