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Specifications
- Antibody type:Primary
- Antigen name:Replication Protein A34
- Clonality:Monoclonal
- Clone:9H8
- Gene ID:6118
- Host:Mouse
- Isotype:IgG1
- Reactivity:Human
- Antigen symbol:RPA34
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- ImmunoPrecipitation:Yes
- Size:500 μl
- Western blot:Yes
- Format:Ig fraction
- Antigen synonyms:HSSB|RPA32|REPA2|Replication Protein A
- Storage buffer:PBS with 1% BSA and 0.05% sodium azide
- Storage temperature:Store at 4-8 °C.
- Immunogen:Full length protein (Human).
- Tested applications:IHC-P, IHC-Fr, IP, WB
- Purification:IgG fraction
- Cat. no.:89351-762
- Supplier No.:GTX22175
Specifications
About this item
RPA is involved in DNA replication, repair and recombination. Human RPA is a stable heterotrimer of 70 kD, 32-34 kD and 11-14 kD subunits (RPA70, RPA32 and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding for SV40 DNA and stimulates DNA polymerase alpha and delta.
IHC: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Type: Primary
Antigen: RPA34
Clonality: Monoclonal
Clone: 9H8
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human