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Specifications
- Antibody type:Primary
- Antigen name:Calpain 2
- Clonality:Monoclonal
- Clone:28F3
- Gene ID:824
- Host:Mouse
- Isotype:IgG1
- Reactivity:Bovine
- Antigen symbol:CAPN2
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoPrecipitation:Yes
- Size:100 μg
- Western blot:Yes
- Antigen synonyms:CANP2|CANPL2|mCANP|FLJ39928|Millimolar-calpain|EC 3.4.22.53|M-calpain|CANPml
- Storage buffer:Preservative: 3mM Sodium Azide. Constituents: 50% Glycerol, 0.15M Sodium chloride, 0.02M Sodium phosphate. pH 7.5.
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1 mg/ml
- Immunogen:Full length protein (Human). Antibody specifically recognizes small subunit.
- Tested applications:ELISA, IP, WB
- Purification:Protein G purified
- Cat. no.:89360-252
Specifications
About this item
Mu Calpain, and m calpain, also known as Calpain 2, are intracellular, calcium dependent cysteine proteases. Mu calpain has a micromolar sensitivity (thus the mu) as compared to the millimolar calcium sensitivity of m calpain. Both Calpains 1 and 2 are composed of an 80 kD subunit and a 30 kD subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. The calpains have papain like activity, thus the pain nomenclature. Both Calpain 1 and Calpain 2 are ubiquitously expressed, and are countered by the endogenous calpain inhibitor, calpastatin.Other calpain family members (calpain 94, ncl2, ncl3, etc) have more limited tissue distribution, and perhaps different functions. The calpain family members consist of a common small subunit (Calpain 4), and a large variable subunit. It is not clear that all calpains contain a small subunit. Domains in the large subunit include the amino terminal domain I, the proteinase domain II, domain III, and the EF hand domain IV. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux.
ELISA: Use at a concentration of 0.5 - 1 ?g/ml. IP: Use at a concentration of 1 - 2 ?g/ml. WB: Use at a concentration of 0.5 - 1 ?g/ml. Predicted molecular weight: 30 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: CAPN2
Clonality: Monoclonal
Clone: 28F3
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human, Bovine