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Specifications
- Antibody type:Primary
- Antigen name:Phosphodiesterase 5A, cGMP-specific
- Clonality:Polyclonal
- Gene ID:171115
- Host:Rabbit
- Isotype:IgG
- Reactivity:Human
- Antigen symbol:PDE5A
- Conjugation:Unconjugated
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:100 μg
- Western blot:Yes
- Storage buffer:Preservative: 0.02% Sodium Azide
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:0.5 mg/ml
- Immunogen:Synthetic cyclic peptide (common to all PDE5A variants) (Rat).
- Tested applications:IP, WB
- Purification:Immunogen affinity purified
- Cat. no.:89367-374
- Supplier No.:GTX14672
Specifications
About this item
The cyclic monophosphate nucleotides (cyclic adenosine monophosphate [cAMP] and cyclic guanosine monophosphate [cGMP]) are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A. The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB PDEs). The cGB PDEs include PDE2, PDE3, PDE5, PDE5 and PDE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimmeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site.
IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects bands of approximately 99 and 89 kDa. (predicted molecular weight: 105 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: PDE5A
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human