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Specifications
- Antibody type:Primary
- Antigen name:Centromere Protein A
- Clonality:Monoclonal
- Clone:3-19
- Gene ID:1058
- Host:Mouse
- Isotype:IgG1
- Neutralisation:Yes
- Reactivity:Human
- Antigen symbol:CENPA
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Size:50 μg
- Western blot:Yes
- Antigen synonyms:centromeric protein A|Histone H3 like centromeric protein A.|CENP-A|centromere protein A|Centromere autoantigen A|CENP A|Centromere protein A 17kDa
- Amino acid number:3 to 19
- Storage buffer:Preservative: None Constituents: 50% Glycerol, PBS. pH 7.2
- Sequence:PRRRSRKPEAPRRRSPS
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1 mg/ml
- Immunogen:Synthetic peptide: PRRRSRKPEAPRRRSPS, corresponding to amino acids 3-19 of Human CENP A.
- Tested applications:ICC/IF, IHC, Neut, WB
- Purification:Protein A purified
- Cat. no.:89359-914
- Supplier No.:GTX13939
Specifications
About this item
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
ICC: Use at a concentration of 10 ?g/ml. IHC: Use at a concentration of 5 ?g/ml. For paraffin embedded sections, heat treatment may be necessary. Microwave sections 2 times for 10 minutes each in citrate buffer (pH 6.5). WB: Use at a concentration of 1 ?g/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. User review: This antibody is great for immunofluorescence on human cells (primary fibroblasts and transformed cell lines such as HeLa, HT1080, A549, H1299). Works well with various fixation conditions, including 1-4% paraformaldehyde (PFA), -20C methanol, 2% PFA followed by -20C methanol, and on unfixed preparations. We routinely use it at a dilution of 1:200-1:400.
Type: Primary
Antigen: CENPA
Clonality: Monoclonal
Clone: 42448
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: