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Specifications
- Antibody type:Primary
- Antigen name:Retinal Pigment Epithelium-specific 65 kDa protein
- Clonality:Monoclonal
- Gene ID:282043
- Host:Mouse
- Isotype:IgG1
- Reactivity:Human
- Antigen symbol:RPE65
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- Size:100 μl
- Western blot:Yes
- Antigen synonyms:sRPE65|all-trans-retinyl-palmitate hydrolase|retinol isomerase|retinoid isomerohydrolase|retinitis pigmentosa 20 (autosomal recessive)|retinal pigment epithelium-specific 65 kDa protein|RBP-binding membrane protein|p63|LCA2|RP20|rd12|mRPE65
- Storage buffer:Preservative: 0.1% Sodium Azide; Constituents: PBS
- Storage temperature:Store at –20 degrees C. Avoid repeated freeze-thaw cycles.
- Concentration:0.96 mg/ml
- Immunogen:Bovine RPE microsomal membrane proteins.
- Tested applications:IHC, WB
- Purification:Protein A purified
- Cat. no.:89359-864
- Supplier No.:GTX13826
Specifications
About this item
Highly conserved among vertebrate species, RPE65 is a major protein of the retinal pigment epithelium (RPE). RPE65 is essential for the regeneration of rhodopsin in the visual cycle. Mutations in RPE65 are responsible for certain forms of autosomal recessive severe retinal dystrophy, including Leber Congenital Amaurosis (LCA). Loss of function results in a block in retinoid processing required for the synthesis of 11-cis retinal and the accumulation of retinyl esters in the RPE.
By Western blot, this antibody recognizes a band at ~65 kDa, representing bovine RPE65 protein. It has also been tested on recombinant human protein in COS7 cells. It may also be used in immunohistochemistry on murine retinal sections and ICC in porcine.Suggested working dilutions: *Western Blot - 1:5,000-1:10,000 (ECL or Alk. Phos.). xpression in primary culture of RPE cells is undetectable in Western blot after day 14. Established cell lines are similarly devoid of RPE65 protein, as indicated in western blot. However, mRNA levels are detectable by Northern for at least 7 weeks. Immunohistochemistry - 1:250 (frozen sections)*The investigator should determine the optimal working dilution for a specific application.
Type: Primary
Antigen: RPE65
Clonality: Monoclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human