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Specifications
- Antibody type:Primary
- Antigen name:Caspase 3
- Clonality:Monoclonal
- Clone:31A1067
- Gene ID:836
- Host:Mouse
- Isotype:IgG1
- Reactivity:Human
- Antigen symbol:CASP3
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- Size:100 μg
- Western blot:Yes
- Antigen synonyms:caspase-3|procaspase3|caspase 3|protein Yama|Yama|cysteine protease CPP32|PARP cleavage protease|CPP32|apoptosis-related cysteine protease|CPP32B|SCA-1|apopain|SREBP cleavage activity 1
- Storage buffer:Preservative: 0.02% Sodium Azide; Constituents: PBS
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:0.5 mg/ml
- Immunogen:Recombinant fusion protein (6xHis tagged full-length human Caspase 3 protein expressed in bacteria).
- Tested applications:WB
- Purification:Protein G purified
- Cat. no.:89359-766
- Supplier No.:GTX13585
Specifications
About this item
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.
WB: Use at a concentration of 2 - 3 ?g/ml. Predicted molecular weight: 31 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: CASP3 (Active + PRO)
Clonality: Monoclonal
Clone: 31A1067
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype:
Reactivity: Human