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Specifications
- Antibody type:Primary
- Antigen name:Crystallin Alpha B
- Clonality:Polyclonal
- Gene ID:1410
- Host:Rabbit
- Isotype:IgG
- Reactivity:Hamster,Pig,Rabbit,Human,Bovine,Rat,Mouse
- Antigen symbol:CRYAB
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:100 μl
- Western blot:Yes
- Antigen synonyms:Heat-shock protein beta-5|HSPB5|CRYA2|Alpha Crystallin B chain|Alpha (B)-Crystallin|CryAB|Rosenthal fiber componet|CTPP2
- Storage buffer:PBS containing 50% glycerol and 0.09% sodium azide
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1 mg/ml
- Immunogen:Synthetic peptide: REEKPAVTAAPKK conjugated to KLH, corresponding to amino acids 163-175 of Human alpha B Crystallin.
- Tested applications:ICC/IF, IP, WB
- Purification:Protein A affinity purified.
- Cat. no.:89359-694
- Supplier No.:GTX13497
Specifications
About this item
Lens proteins consist almost entirely of Crystallins (about 95%). Crystallins are also found in vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha Crystallin is the largest of the crystallins and is composed of 2 primary gene products; alpha A and alpha B. There are at least 5 different proteins comprising the beta Crystallins. The gamma Crystallins are monomeric, but there are at least 5 gamma Crystallins identified in bovine and rat lens. Alpha Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha Crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha B Crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
Recommended Starting Dilutions:ICC: Use at a dilution of 1/200. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000 - 1/2000. 1/2000 dilution is sufficient to detect 50ng of alpha B Crystallin. Detects a band of approximately 22 kDa (predicted MW 20 kDa).
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: CRYAB
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: