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Specifications
- Antibody type:Primary
- Antigen name:RAs-related Nuclear protein
- Clonality:Monoclonal
- Clone:ARAN1
- Gene ID:5901
- Host:Mouse
- Isotype:IgG2b
- Reactivity:Bovine
- Antigen symbol:RAN
- Conjugation:Unconjugated
- ELISA:Yes
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:50 μl
- Western blot:Yes
- Antigen synonyms:OK/SW-cl.81|GTP-binding nuclear protein Ran|RAN|ARA24|GTPase Ran|Androgen receptor-associated protein 24|Ras-like protein TC4
- Storage buffer:Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:2 mg/ml
- Immunogen:Denatured recombinant protein (Human).
- Tested applications:ELISA, ICC/IF, IP, WB
- Purification:Protein A purified
- Cat. no.:89359-566
Specifications
About this item
Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in Ran disrupt DNA synthesis. Because of its many functions, it is likely that Ran interacts with several other proteins. Ran regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. Ran could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of Ran-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Ran is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). Ran coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.
ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 25 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: RAN
Clonality: Monoclonal
Clone: ARAN1
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b
Reactivity: Bovine