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Specifications
- Antibody type:Primary
- Antigen name:Peroxisome Proliferator-Activated Receptor Alpha
- Clonality:Monoclonal
- Clone:3B6/PPAR
- Gene ID:5465
- Host:Mouse
- Isotype:IgG2b
- Reactivity:Human
- Antigen symbol:PPARA
- Conjugation:Unconjugated
- Flow cytometry:Yes
- ImmunoPrecipitation:Yes
- Size:50 μg
- Western blot:Yes
- Antigen synonyms:Nr1c1|PPAR alpha|4933429D07Rik|AW742785|Ppar|PPAR-alpha
- Storage buffer:PBS containing 0.05 % sodium azide
- Storage temperature:–20 °C, avoid freeze/thaw cycles
- Concentration:1 mg/ml
- Immunogen:Purified recombinant PPAR alpha protein.
- Tested applications:IP, WB
- Purification:purified
- Cat. no.:89359-368
- Supplier No.:GTX12412
Specifications
About this item
Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.
Recommended Starting Dilutions:FACS: 1µg per 10^6 cellsIHC: 1:200WB: 1:100 - 1:500Not tested in other applications. Optimal dilution / concentration should be determined by the end user.
Type: Primary
Antigen: PPARA
Clonality: Monoclonal
Clone: 3B6/PPAR
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b
Reactivity: Human