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Specifications
- Antibody type:Primary
- Antigen name:KDEL receptor
- Clonality:Monoclonal
- Clone:KR-10
- Gene ID:618184
- Host:Mouse
- Isotype:IgG1
- Reactivity:Bovine
- Antigen symbol:KDEL Receptor
- Conjugation:Unconjugated
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Size:50 μl
- Western blot:Yes
- Storage buffer:None
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Immunogen:Synthetic peptide conjugated to KLH, corresponding to amino acids 192-212 of Cow KDEL Receptor.
- Tested applications:ICC/IF, IP, WB
- Purification:Unpurified
- Cat. no.:89359-286
Specifications
About this item
Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product.
For ICC: Use at a dilution of 1:900 - 1:1,100. For IP: Use at a dilution of 1:400 - 1:600. For WB: Use at a dilution of 1:200 - 1:300. Predicted molecular weight: 25 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Type: Primary
Antigen: KDEL Receptor
Clonality: Monoclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Bovine