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Specifications
- Antibody type:Primary
- Antigen name:High-mobility Group Box 1
- Clonality:Monoclonal
- Clone:HAP46.5
- Gene ID:3146
- Host:Mouse
- Isotype:IgG1
- Reactivity:Dog
- Antigen symbol:HMGB1
- Conjugation:Unconjugated
- ELISA:Yes
- Size:100 μl
- Western blot:Yes
- Antigen synonyms:high-mobility group box 1|RP11-550P23.1|high mobility group protein 1|Amphoterin|SBP-1|high-mobility group (nonhistone chromosomal) protein 1|high mobility group protein B1|HMG3|Sulfoglucuronyl carbohydrate binding protein|HMG1|HMG-1
- Storage buffer:Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:1.9 mg/ml
- Immunogen:Recombinant full length protein (Human).
- Tested applications:ELISA, WB
- Purification:Protein A purified
- Cat. no.:89359-110
- Supplier No.:GTX12029
Specifications
About this item
HMG1 and HMG2, chromatin non-histone high mobility group proteins 1 and 2, are 27 and 25 kDa members of a family of proteins containing multiple HMG-boxes, conserved domains of 80 amino acids which mediate DNA binding of many proteins. HMG box domains recognize DNA structure, such as prebent, supercoiled or four way junction DNA, and non- specific DNA sequences. Both HMG1 and HMG2 contain an N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of these proteins contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMG1/2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites, such as steroid hormone receptors, p53, and Oct. HMG1/2 may serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription and replication.
ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.5 - 1 ?g/ml. Detects a band of approximately 25 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: HMGB1
Clonality: Monoclonal
Clone: HAP46.5
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Dog