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Specifications
- Antibody type:Primary
- Antigen name:Actin Alpha 1, Skeletal Muscle
- Clonality:Monoclonal
- Clone:AC-40
- Gene ID:58
- Host:Mouse
- Isotype:IgG2a
- Reactivity:Carp
- Antigen symbol:ACTA1
- Conjugation:FITC (Fluorescein Isothiocyanate)
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Size:50 μl
- Antigen synonyms:ACT1|alpha skeletal muscle Actin|ASMA|alpha Actin 1|NEM2|ACTA|NEM1
- Storage buffer:Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4
- Sequence:SGPSIVHRKCF
- Storage temperature:Keep as concentrated solution. Store at 4 °C short term. For extended storage aliquot and store at –20 °C or below. Avoid freeze-thaw cycles.
- Concentration:2.8mg/ml
- Immunogen:Synthetic peptide: SGPSIVHRKCF (human) attached to a Multiple Antigen Peptide (MAP) backbone.
- Tested applications:ICC/IF, IHC-P, IHC-Fr
- Purification:Protein A purified
- Cat. no.:89356-164
- Supplier No.:GTX11005
Specifications
About this item
Mouse monoclonal [AC - 40] to Actin (FITC)
IF: Use at a dilution of 1/50 (determined by direct immunofluorescent labeling of cultured human fibroblasts). The conjugate is suitable for dual immunofluorescent staining procedures. IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. Direct Immunofluorescent Labeling of Cultured Cells:Materials:1. Coverslips.2. Cells (e.g., human or chicken fibroblasts) in DMEM medium + 10% Fetal Calf Serum 3. 10 mM phosphate buffered saline (PBS), pH 7.2-7.4, without preservative 4. PBS containing 1% BSA (diluent).5. Absolute methanol cooled to ?20 °C.6. Acetone, Analytical grade, cooled to ?20 °C (1:1 v/v).7. Aqueous mounting medium.8. Test antibody conjugateCell Growth and Fixation:1. Collect cells from tissue culture dish at a stage of almost confluency, wash with medium and seed on to coverslips. Seed 1-2 x 10^4 cells per cover slip and grow cells in incubator for 2-3 days. Do not change medium.2. Remove coverslips from incubator, discard medium.3. Wash twice with PBS, remove solution by aspiration.4. Add enough cold methanol to cover the cell layer. Incubate 10 minutes at ?20 °C. Aspirate solution.5. Cover cell layer with cold acetone. Incubate 1 minute at ?20 °C. Aspirate solution.6. Wash twice with PBS. Rehydrate in PBS for at least 30 minutes prior to labeling with antibody. Test:1. Dilute Monoclonal Anti-Actin FITC Conjugate 1:50 in PBS containing 1% BSA (diluent). Add enough diluted antibody to cover the cell layer and incubate coverslip for 60 minutes at room temperature.2. Wash 3 times with PBS, 5 minutes each.3. Drain excess solution by touching edge of coverslip on paper toweling.4. Invert onto mounting medium applied on glass slides.5. Read under the UV fluorescence microscope. Mounted preparations can be stored in the dark at 2-8 °C. Note:1. Do not allow cell layer to dry out at any time during the procedure.2. In case of excessive background staining remove aggregates from the labeled reagent (conjugate) by centrifuging for 15 minutes immediately prior to use. The antibody can be used for staining of methanol-fixed, frozen sections. The epitope recognized by the antibody is resistant to formalin fixation and paraffin-embedding. The conjugate may also be used for the localization of actin using direct immunofluorescent staining of frozen or fixed tissue sections and cultured cells. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: ACT1
Clonality: Monoclonal
Clone: AC-4
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a
Reactivity: Carp