About this item
The Total RNA Mini and Maxi Kits for Plants provide a simple and fast method to isolate total RNA from plant tissue and cells
Samples are groomed in liquid nitrogen and filtered to remove debris. In the presence of a binding buffer and chaotropic salt, the total RNA in the lysate binds to the glass fiber matrix of the spin column. The optional DNase treatments can remove DNA residues and the contaminants can be washed with an ethanol based wash buffer. Finally, the purified total RNA is eluted by RNase-free water. This protocol does not require phenol extraction or alcohol precipitation, and the entire procedure can be completed within 60 minutes. The purified total RNA is ready for RT-PCR and Northern Blotting.
Sample size: Up to 100 mg of Fresh Plant Tissue, 25 mg of Dry Plant Tissue.
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