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Specifications
- Description:GlycoLink Micro Immobilization Kit
- Size:10-column kit
- Cat. no.:CAPI88942
- Supplier No.:88942
- Sufficient For:Immobilizing 50 to 500 µg of glycoprotein per column
Specifications
About this item
Hydrazide-activated resin to immobilize glycoproteins for affinity purification.
- High-capacity—immobilize 1 to 10mg of oxidized antibody or other glycoprotein per milliliter of resin
- Fast and efficient—immobilize in as few as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours (efficiency depends on the amount and type of glycosylation)
- Specific immobilization—the hydrazide-activated UltraLink Resin conjugates only to purified glycoproteins whose sugar (e.g., sialic acid) groups have been gently oxidized with periodate
- Stable attachment—resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilization step is required
- Preserves antibody function—immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target
- Convenient sizes—choose from two complete 10-column kits: one with 1mL columns and one with 0.1mL columns
Thermo Scientific GlycoLink Immobilization Kits provide affinity columns and reagents to immobilize glycoproteins through oxidized sugar groups for use in affinity-purification applications or studies of protein-protein interactions.
The GlycoLink Kits use hydrazide-activated resin and a special catalyst to prepare affinity columns with polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions. Any glycosylated proteins (even monoclonal antibodies) that contain periodate-oxidizable carbohydrate groups can be immobilized with these kits to make reusable affinity columns. Antibodies immobilized by this hydrazide immobilization chemistry have unobstructed antigen-binding sites and optimal purification capability. Prepared columns with stable glycoproteins or antibodies can be regenerated and reused at least five times for affinity-purification without significant loss in binding capacity.
Kit Content for PI88941:
GlycoLink Columns, 1mL, 10 columns
GlycoLink Coupling Buffer, 2 x 100mL
Aniline, 0.2mL
Wash Buffer, 120mL
Sodium meta-periodate, 0.5g
Zeba Spin Desalting Columns, 5mL, 10 columns
PBS Pack (makes 500mL), 1 pack
Column Accessories, 10 ea
Kit Content for PI88942:
GlycoLink Columns, 0.1mL, 10 columns
GlycoLink Coupling Buffer, 100mL
Aniline, 0.2mL
Wash Buffer, 60mL
Sodium meta-periodate, 0.5g
Zeba Spin Desalting Columns, 0.5mL, 10 columns
PBS Pack (makes 500mL), 1 pack
Column Accessories, 10 ea
Kit Content is N/A for PI53149.