Hamilton Company offers one of the most comprehensive selections of chromatography columns in the industry.

• Separation of Proteins, Peptides, and Enhanced Recovery of Oligonucleotides
• pH Stable from 1 to 13
• Excellent Durability (Stable to any Concentration of Water or Organic Solvent)
• Lower Back Pressure Operation Compared to Other Polymer HPLC Columns
• Custom Hardware Dimensions Available
• Buffer strength 0.0 to 0.5N

Each column is manufactured under rigorous criteria and tested for resolution and retention time accuracy.

Hamilton PRP-h5 reversed phase columns are ideal for applications that require a high degree of column-to-column and batch to batch consistency (quality assurance, clinical trials). Unlike C8 or C18 columns, PRP-h5 has no stationary phase coating. The integral reversed phase characteristics of the PRP-h5 column eliminate the need for special coating techniques. Since there is no stationary phase to hydrolyze, the column maintains its performance characteristics longer than many C8 or C18 columns. While many column manufacturers rely on other sources for their raw silica support, Hamilton manufactures its PRP-h5 support, thus assuring a consistent supply of PRP-h5 columns.

PRP-h5 columns are packed with a poly(styrene-divinylbenzene) material. This support is pressure stable up to 5000 psi, and highly cross-linked to prevent shrinkage or swelling (common in other polymeric columns). Since the support is polymeric, column lifetime is longer for PRP-h5 than C4, C8 or C18 columns. Regardless of whether you use ion-pair reagents or high percentage aqueous buffers (80 to 100%) for your separation, PRP-h5 columns outlast silica-based columns under most conditions. PRP-h5 columns will last approximately one year, while C8 and C18 columns will last only 3 to 4 months.

Housing Material: Anodized Aluminum
Connecting Tubing Size/Material: ¹/₁₆" ext.Ø., 0.28 mm int.Ø/316 Stainless Steel
Cartridge Materials,
Barrel: CTFE
Frits: 316 Stainless Steel
Sleeve: 304 Stainless Steel
Frit Porosity: 2 µm
Cartridge Dimension: 20×2.3 mm int.Ø
Cartridge Bed Volume: 80 µl
Chemical Compatibility: Recommended for use with virtually all solvents except high concentrations of sodium chloride.