Hamilton Company offers one of the most comprehensive selections of chromatography columns in the industry.

• pH Stable from 1 to 13
• Better Sample Recovery than Silica Based Columns
• Excellent Durability
• Eight Available Column Int.Ø: 1.0 to 50.8 mm
• Two Column Materials Available: 316 Stainless Steel and PEEK
• Analytical and Semiprep/Preparative Guard Columns
• Buffer strength 0.0 to 0.5N

Each column is manufactured under rigorous criteria and tested for resolution and retention time accuracy.

PRP-3 polymeric reversed phase columns last longer than silica-based columns. The columns are packed with a poly(styrene-divinyl)benzene support. The support is pressure stable up to 5000 psi and crosslinked to prevent shrinkage or swelling (common in other polymeric HPLC columns) when the mobile phase is changed. Since the support is entirely polymeric and there are no C18 groups to hydrolyze, column lifetime is longer. Regardless of whether you use ionpair reagents or high percentage aqueous buffers (80 to 100%) for your separation, PRP-3 columns outlast silica-based columns under common (pH 2.0) or uncommon (pH 12.7) conditions.

While most reversed phase columns are slowly destroyed by buffers below pH 2.0 or above 8.0, the inherent stability of PRP-3 columns allows their use for any protein purification method from pH 1 to 13. This is very helpful for proteins that are unstable or have poor solubility at a pH below 8.0.

PRP-3 columns are easy to use and ideal for almost any protein separation, including pH conditions from 1 to 13, with organic modifier (0 to 100%) of virtually any type from routine to the unusual. A selection of column sizes are available from analytical to semi-prep and preparative scale. Sample scaleup is easy because the PRP-3 packing is consistent from analytical to preparative columns. This saves time and eliminates the need to redevelop your separation on semi-prep or preparative columns.