Hamilton Company offers one of the most comprehensive selections of chromatography columns in the industry.

• pH Stable from 1 to 13
• Polymeric Columns for General Separations
• Four Particle Sizes: 5, 7, 10, and 12 to 20 µm
• Custom Hardware Dimensions Available
• Analytical and Semiprep/Preparative Guard Columns
• Buffer strength 0.0 - 0.5N

Each column is manufactured under rigorous criteria and tested for resolution and retention time accuracy.

PRP-1 polymeric HPLC columns with 100 Å pore size particles are designed for broad ranging uses, similar to C8 or C18 HPLC materials. Hamilton PRP-1 (Polymeric Reversed Phase) HPLC columns combine the best characteristics of silica-based and polymeric columns to arrive at a column that is highly inert and long-lasting. Quality assurance as well as research and development laboratories find that PRP-1 columns last significantly longer than silica-based C8 or C18 columns. This is particularly true for difficult analyses such as high pH samples (pH 8 to 13), labile or reactive samples (irreversible adsorption), high aqueous purifications (80 to 100% water), and separations with ion pairing reagents.

Stability from pH 1 to 13 allows for the analysis of samples at high pH (8 to 13) without the possibility of column degradation or loss of efficiency. An expanded pH range also allows samples to be chromatographed while ionized.

Sample Recovery is vital to sample purification efforts. Problems arise when labile sample become irreversibly attached to the silanol groups present on C8 and C18 columns. Since PRP-1 is made entirely of polystyrene-divinylbenzene, there are no silanol groups to cause sample loss. Thus, the recovery and quantitation of labile and reactive samples is enhanced.

PRP-1 columns are packed with a poly(styrene-divinylbenzene) material. This support is pressure stable up to 5000 psi, and cross-linked to prevent shrinkage or swelling (common in other polymeric columns). Since the support is entirely polymeric with no silica to deteriorate, column lifetime is longer for PRP-1 than silica C4, C8 or C18 columns.

Housing Material: Anodized Aluminum
Connecting Tubing Size/Material: ¹/₁₆" Out.Ø, 0.28 mm Int.Ø/316 Stainless Steel
Cartridge Materials,
Barrel: CTFE
Frits: 316 Stainless Steel
Sleeve: 304 Stainless Steel
Frit Porosity: 2 µm
Cartridge Dimension: 20×2.3 mm Int.Ø
Cartridge Bed Volume: 80 µl
Particle Size: 12 to 20 µm for Polymeric packings, 10 µm for Silica supports
Chemical Compatibility: Recommended for use with virtually all solvents except high concentrations of sodium chloride.