G-Biosciences' Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose

The normal biotin-avidin complex requires strong denaturing buffers, i.e. 8 M guanidineHCl, to denature the avidin and release the biotin, which obviously destroys the native and functional aspects of the avidin.

G-Biosciences' Iminobiotin Resin does not require strong, denaturing elution buffers. The iminobiotin-avidin complex will form at >pH 9.5 and can be dissociated at pH 4.0 with gentle elution buffers, including 50 mM ammonium acetate, pH 4.0 with 0.5 M NaCl. The biotin binding capacity is >2 mg avidin per mL resin.

The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non-denaturing elution buffers.