G-Biosciences' Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase

G-Biosciences’ Pfu DNA Polymerase 2X Mastermix is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs, MgSO₄ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Pfu Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination.

UNIT DEFINITION: One unit (U) of Taq polymerase is defined as the amount of enzyme needed to catalyze the incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 70°C using herring sperm DNA as a substrate.

STORAGE BUFFER: 20 mM Tris.HCl (pH8.0), 100 mM KCl, 3 mM MgCl₂, 1 mM DTT, 0.1% Nonidet® P-40, 0.1% Tween® 20, 0.2 mg/mL BSA, 50% (v/v) glycerol.

10X Pfu BUFFER: 200 mM Tris-HCl (pH8.8), 100 mM KCl, 100 mM (NH₄)₂SO₄, 20 mM MgSO₄, 1% Triton® X-100 and 1 mg/mL BSA.

Its molecular weight is 90 kD. It can amplify DNA target up to 2 kb. The elongation velocity is 0.2-0.4 kb/min (70-75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.