About this item
Trypsin is a serine endopeptidase that specifically cleaves peptide bonds that are readily identified by mass spectrometry.
- The resulting trypsin is extremely resistant to autolysis and has a specific activity over 10,000 units/mg protein
- The maximum activity is in the pH range of 7 to 9 and the activity is reversibly inactivated at pH 4
Native trypsin is prone to autolysis that results in pseudotrypsin, which exhibits a broader proteolytic specificity and trypsin fragments that interfere with sequence analysis.
Unlike other trypsin preparations, our Mass Spectrometry Grade Trypsin is highly stable, and as a result can be stored for a long period without any loss of activity. For convenience, Mass Spectrometry Grade Trypsin (porcine) is supplied lyophilized as 100 or 20µg vials with a specific resuspension buffer.
In addition, the modified trypsin is TPCK treated to inactive the interfering chymotrypsin activity and the resulting protein is affinity purified and lyophilized.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.