About this item
ProteaseArrest™ is a general protease inhibitor cocktail solution that is provided as a 100X concentrated, ready-to-use solution
ProteaseArrest™ Outperforms Tablet Cocktails: The ProteaseArrest™ format allows delivery of optimized concentrations of protease inhibitor, for example 2X or higher concentrations can be added for tissues with higher than normal protease concentrations; a feature not possible with tablet format protease inhibitor cocktails. In our study, a 1X concentration of ProteaseArrest™ inhibits over 95% of protease activities (e.g. 0.5mg/ml Mouse Pancreas Extract). The ProteaseArrest™ protease inhibitor cocktails demonstrate greater inhibition levels compared to similar protease inhibitor cocktails, including tablet formats. In independent studies, researchers have found that ProteaseArrest™ outperforms several leading manufacturer's protease inhibitor cocktails, including tablet formats, in the purification of plant proteins.
ProteaseArrest™ shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins during preparation of samples and protein purification from animal tissues, plants, yeast, bacteria, and fungal lysates. For enhanced inhibition, ProteaseArrest™ is supplied in species specific formats for plant, bacteria, mammalian, yeast, fungal and a specialized recombinant protein purification inhibitor. These cocktails are based on ProteaseArrest™ but are enhanced with specialized inhibitors. ProteaseArrest™ is also available as single use aliquots that are suitable for >95% protease inhibition in 10ml solutions. ProteaseArrest™ is also offered as a dry format, called ProteCEASE™, for those who prefer reconstitution prior to use. For protection of proteins from proteolysis in such applications as 2D electrophoresis, we offer FOCUS™ ProteaseArrest™.
It is suitable for small, analytical sample applications, as >95% inhibition is achieved by adding 10µL ProteaseArrest™ per mL sample. For samples with higher than normal protease levels, the volume of ProteaseArrest™ added can be increased for greater inhibition levels. Protease levels can easily be assayed with our Protease Assays. The cocktail contains reversible and irreversible inhibitors of serine, cysteine, calpain and metallo- proteases. An optional EDTA solution is provided for enhanced metalloprotease inhibition. It is not present in the actual ProteaseArrest™ cocktail as it would inhibit the activity of proteins that require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity. In addition, EDTA will inhibit the purification of proteins using immobilized metal affinity chromatography (IMAC).
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.