The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample

The kit is supplied with our chemically stabilized MSG-Trypsin™ for use as a general protease control; however, other specific protease standard controls can be used. MSG-Trypsin™ is an ultra-pure trypsin from porcine pancreas, modified by methylation followed by TPCK treatment and is extremely resistant to autolysis. The kit components are sufficient for 1,000 assays in a microtiter plate format.

Accessories: Resorufin-Casein Protease Substrate (#786-321): A colorimetric substrate that when treated with proteases releases resorufin that has an absorbance of 570nm. Supplied lyophilized. FITC-Casein Protease Substrate (#786-322): A fluorescent (fluorescein isothiocyanate (FITC)) substrate that when treated with proteases releases FITC that has an excitation at 485nm and emission at 535nm. Supplied lyophilized.

The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485nm and emission at 530nm to determine protease activity. The kit detects picogram level of proteases present in the sample.