To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Description:TERRIFIC BROTH, POWDER
- Size:500 g
- Cat. no.:97063-418
- Supplier No.:J869-500G
Specifications
About this item
Terrific Broth is an enriched medium, ideal for culturing cells for nucleic acid purification and recombinant protein expression.
- Save time using premixed and ready-to-use media
- Homogenous and high quality powders
It is an enriched medium containing high levels of tryptone and yeast extract, which provide amino acids, vitamins and trace elements necessary for high density growth. Glycerol is added as a carbohydrate source, while the potassium phosphate is present to both maintain optimal pH and serve as a source of potassium for cellular metabolism.Glycerol is not supplied with this media.
Suspend 47.6 g of Terrific Broth powder and 4 mL of glycerol in 1 L of purified water and allow to dissolve.Autoclave for 15 minutes at 15 psi at 121°C. Note: Alternatively, medium may be sterile-filtered.Allow the solution to cool to 50°C or below before adding supplemental components and antibiotic.
1L of prepared media contains 12 grams of tryptone, 24 grams of yeast extract, 9.4 grams of potassium phosphate dibasic, 2.2 grams of potassium phosphate monobasic.
Store Terrific Broth, Powder at room temperature (18 – 26°C). Store prepared medium cold (2 – 8°C).