Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications

• Fast, efficient transfer in 10–20 minutes
• Utilizes standard transfer equipment
• Non-hazardous formulation
• Transfers to PVDF and nitrocellulose from multiple types of gels

Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively. Rapid Transfer Buffer is a methanol-free, non-hazardous formulation that is compatible with PVDF and nitrocellulose membranes. It is used for transfer of proteins from standard Laemmli gels, commercial pre-cast gels, and VWR’s NEXT GEL™. The transfer efficiency is equivalent to that observed when using a Tris-Glycine-Methanol transfer buffer.