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DEAE Sephacel™ Ion Exchange Chromatography Media, Cytiva
DEAE Sephacel™ Ion Exchange Chromatography Media, Cytiva
Catalog #: CA95016-862L
Supplier:  Cytiva
CAS Number:  
DEAE-SEPHACEL 500 ML
DEAE Sephacel™ Ion Exchange Chromatography Media, Cytiva
Catalog #: CA95016-862L
Supplier:  Cytiva
CAS Number:  

Specifications

  • Particle size:
    100 μm
  • pH range:
    2 - 12
  • Description:
    DEAE Sephacel™
  • Flow rate:
    <50 cm/h
  • Size:
    500 mL
  • Storage temperature:
    4 - 8 °C
  • Binding capacity:
    10 mg Thyroglobulin (MW 669 000)/mL drained medium, 160 mg HSA (MW 68 000)/mL drained medium
  • Total ionic capacity:
    0.10–0.14 mmol Cl-/mL
  • Cat. no.:
    CA95016-862L
  • Type:
    Liquid
  • Exclusion Limit:
    1000000 daltons
  • Supplier No.:
    17-0500-01

Specifications

About this item

DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.

The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of approximately 1,000,000 daltons for globular proteins. Supplied pre-swollen in 20% ethanol. Autoclavable in 0.1 M NaCl at 121°C for 30 min. Avoid using oxidizing materials. Stable in aqueous buffers, 1.0 M acetic acid, 1.0 M NaOH, 8 M Urea, 8 M guanidine hydrochloride, ethanol, methanol. Negligible volume variation due to changes in pH or ionic strength.

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