About this item
The bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high quality genomic DNA from gram-positive and gram-negative bacteria. The kit can be used to isolate genomic DNA from a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis, and more. The illustra™ bacteria genomicPrep Mini Spin Kit produces highly pure and intact genomic DNA with A260/A280 values of 1.75 - 1.85.
genomicPrep™ kits are designed for the purification of high-quality genomic DNA from a variety of sources. Specific kits are available for blood, tissue and cells, as well as bacteria, and are validated in a number of downstream applications including RT-PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests. Protocols minimize DNA shearing during purification, resulting in more intact, higher molecular weight DNA. Kits also feature a greater input capacity and take less time than comparable kits. Midi flow kits replace isopropanol precipitation with a much simpler 15-minute column desalting step, saving hours of time. Blood genomicPrep™ kits can be used to purify DNA from whole blood, buffy coat, bone marrow, and nucleated red blood cells. tissue and cells genomicPrep™ kits are designed to work with a variety of animal tissues and mammalian cell cultures. Bacteria genomicPrep™ mini spin kits will extract DNA from either gram-positive or gram-negative bacteria.
- Total DNA extraction time under one hour with reduced lysis time
- Can be used to purify 4 to 8 μg of genomic DNA from 2×10⁹ bacteria cells with input amounts ranging from 1×10⁹ to 4×10⁹ bacteria cells
- Spin columns, prepacked with a silica membrane, provide excellent reproducibility and consistency
- Verified in several downstream applications, including Real-Time PCR, long PCR, and restriction digest
- Color coded caps for ease of use
The bacteria genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high quality genomic DNA from gram-positive and gram-negative bacteria. The kit can be used to isolate genomic DNA from a wide range of bacterial strains such as DH5a, TOP10, JM109, Bacillus subtilis, and more. The illustra™ bacteria genomicPrep Mini Spin Kit produces highly pure and intact genomic DNA with A260/A280 values of 1.75 - 1.85.
Different elution volumes can be used to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, Real-Time PCR, and long PCR. The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.
Different elution volumes can be used to isolate genomic DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, Real-Time PCR, and long PCR. The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.
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