The Monarch spin PCR and DNA cleanup kit (5 μg), a newer version of our original PCR cleanup kit, offers an efficient method for purification and concentration of up to 5 μg of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription.

• Achieve high yields (up to 5 μg) and high purity in PCR, DNA and oligo cleanup and purification
• Elute in very small volumes, in as low as 5 μl for elution, for highly concentrated DNA
• Protocol for oligo cleanup, enabling purification of ssDNA, oligo, and other small DNA fragments
• Features enhanced column design and optimized buffer system without the need to adjust pH
• Designed for sustainability with less plastic used in columns and kit compared to leading suppliers

This new version features upgraded spin columns precision-engineered to allow low elution, in as little as 5 μl, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations.

The optimized gel dissolving buffer allows efficient gel dissolving and DNA binding on the column, without a need to monitor pH or add isopropanol. These kits contain columns and bottles designed to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps.