BT97 super rapid self-contained biological indicator for monitoring of airborne and surface disinfection by hydrogen peroxide (H₂O₂) 10⁶ Geobacillus stearothermophilus ATCC 7953 spores per vial.

• Biological indicator to verify room disinfection
• Consistent and reliable spore population and D-value
• Sensitivity >97%
• Shelf-life: 2 years
• Must be read with Bionova Minibio autoreader (VWR part 77506-948)
• D-Value is provided at 50 ºC , 2 mg/L H₂O₂ and according to manufacturer test conditions

Each box of 50 tubes allows 25 detections: 1 spore tube + 1 culture medium tube. Readout: 1 hour. Bionova® BT97 BioSurf biological indicator has been designed for quick and easy monitoring of airborne and surface disinfection processes by hydrogen peroxide.

The system consists of two tubes: tube A which contains the spores to challenge the disinfection process and tube B which is used to reveal its success.

An optional visual pH color change confirmation could be performed after 48 hours of incubation. If disinfection process has not been successful, culture medium will change to yellow during incubation at 60 °C, thus showing the presence of living spores. If disinfection process is successful culture medium will remain purple after the incubation process. 7-day readout is optional and not intended to be routinely performed; it is an initial validation of the 1-hour reading. Fluorescence results may be compared to the 7-day visual reading. NOTE: If 7-day readout is performed, a humidified environment will be required to prevent medium from drying out.

Tube A: Biological indicator with spores carrier.
Tube B: Culture medium,: 0.5 to 0.7 ml ampoule.

Storage conditions:
T = 10 to 30 °C, RH = 30 to 80%, keep the product away from direct light.