Spore coupon biological indicator for plasma or vaporized hydrogen peroxide in single glassine packaging. Geobacillus stearothermophilus (ATCC 7953) 10⁶ spores per coupon. Readout in 24 h. It is suggested the use of Bionova® MC1020-2 culture medium.

• Consistent and reliable spore population and D-value
• Envelope size: 25×70 mm non-absorbent material package
• Spores carrier inside: Stainless steel coupon 34.0×7.0×0.8 mm
• D-Value is provided according to manufacturer test conditions
• Shelf-life: 2 years
• Separate culture medium is required
• Culture medium MC1020-2 (VWR# 77506-994) and incubation in Bionova Dual Incubator IC10/20 recommended (VWR part 77506-948)

The envelope containing Bionova® BT93 spore coupon is specifically designed to control plasma or vaporized hydrogen peroxide sterilization processes. Spore coupons should be incubated in MC1020-2 Bionova® growth medium tube or other medium appropriate for Geobacillus stearothermophilus ATCC® 7953 growth.

f sterilization process was not effective, MC1020-2 growth medium will turn to yellow after incubation between 55 to 62 °C, thus indicating the existence of living Geobacillus stearothermophilus spores in the spore coupon.

If sterilization process was effective, MC1020-2 growth indicating medium will remain its original color. Final readout should be done after a 24-hour incubation between 55 to 62 °C of the growth medium containing the spore coupon.

For conventional culture media like TSB, incubation for 7 days between 55 to 62 °C is recommended

Storage conditions:
T = 10 to 30 °C, RH = 30 to 80%