Rapid self-contained biological indicator for steam sterilization processes. Geobacillus stearothermophilus (ATCC® 7953) 10⁶ spores per vial. Readout: 3 hours.

• Consistent and reliable spore population and D-value
• Sensitivity >97%
• Shelf-life: 2 years
• Must be read with Bionova Minibio autoreader (VWR part 77506-948)
• D-Value: not lower than 1.5 minutes at 121 °C, another D/value is informed at 132 and 135 °C
• Z-value is informed

A visual confirmation of the result is optional (color change of the medium caused by pH change) and can be made after incubation at 60 °C for 48 hours and/or for 7 days.

If sterilization process has not been successful, culture medium will change to a greenish color first, and then to yellow, thus showing the presence of living spores. If sterilization process is successful culture medium will remain purple after the incubation process.

7-day readout is optional and not intended to be routinely performed; it is an initial validation of the 3-hour reading. Fluorescence results may be compared to the 7-day visual reading.

Storage conditions:
T = 10 to 30 °C, RH = 30 to 80%, keep the product away from direct light.