Rapid self-contained biological indicator for ethylene oxide sterilization processes. Bacillus atrophaeus (ATCC® 9372) 10⁶ spores per vial. Readout: 4 hours.

• Consistent and reliable spore population
• Culture medium: 0.5 to 0.7 ml, blue color
• Shelf-life: 2 years
• Must be read with Bionova Minibio autoreader (VWR part 77506-948)

An optional visual pH color change confirmation could be made after 48 hours of incubation. If sterilization process has not been successful, culture medium will change to a greenish color first, and then to yellow during incubation at 37 °C, thus showing the presence of living spores. If sterilization process is successful culture medium will remain blue after the incubation process. Please review the instructions for use for applicable cycles in your country

7-day readout for visual confirmation by color change is optional and not intended to be routinely performed; it is an initial validation of the 4 hour-reading. Fluorescence results may be compared to the 7-day visual reading.

NOTE: if 7-day readout is performed, a humidified environment will be required to avoid medium dry out.

Storage conditions:
T = 10 to 30 °C, RH = 30 to 80%, keep the product away from direct light.