PS, sterile. The Corning® CellSTACK® culture chambers are available in five sizes and with three different surface treatments.

• Two 26 mm diameter filling ports allow direct access to chamber bottom providing greater flexibility for sterile filling and emptying by pouring, pipetting or via tubing in a fully closed system
• Standard 33 mm threaded caps have 0.2 μm pore non-wettable membranes sealed directly to the caps to allow gas exchange while minimising the risk of contamination
• Optional 33 mm threaded caps are available with integrally sealed USP Class VI certified C-Flex® tubing to allow direct sterile transfer of media and cells via pumping or gravity feed
• Polystyrene construction provides excellent optical clarity and mechanical strength

TC treated surface: Uncharged polystyrene has an uncharged, hydrophobic surface to which cell attachment proteins bind poorly. This results in poor and uneven cell attachment and growth. Tissue culture (TC) treated polystyrene has a negatively charged, hydrophilic surface to which cell attachment proteins bind evenly. This provides a good surface for cell attachment and growth.

Other sterile 33mm threaded caps are also available: a solid unvented cap for closed systems or in applications where venting is not necessary; vented filling caps with integrally sealed USP Class VI certified 9.5mm (³/₈") I.D. C-FLEX® tubing and fitted with a 50mm filter and 0.2µm pore membrane; vented filling caps with integrally sealed USP Class VI certified 6.4mm (¹/₄") I.D. tubing and fitted with a Air Vent Filter, for use when increased venting is required; and universal over caps for use with existing tubing systems. The stacking device (66025-658) models the top surface of the chamber, securely nesting in the strengthening ribs. It is used to optimize incubator space while providing clearance for venting. Chambers are manufactured under GMP conditions and 100% leak-tested prior to shipping. Certified nonpyrogenic and sterilized by gamma irradiation.

Corning® CellBIND® surface: The Corning® CellBIND® surface enhances cell attachment under difficult conditions, such as reduced-serum or serum-free medium, resulting in higher cell yields.
More consistent cell attachment resulting in increased cell growth
Adapts cells more quickly to reduced-serum or serum-free conditions
Reduces premature cell detachment from confluent cultures
May eliminate the need for tedious, time-consuming, low stability biological coatings
Requires no refrigeration or special handling and is stable at room temperature

Ultra-Low Attachment surface: The Ultra-Low Attachment surface is a covalently bonded hydrogel surface that is hydrophilic and neutrally charged. It minimises cell attachment, protein absorption and enzyme activation. The surface is non-cytotoxic, biologically inert and non-degradable.
Maintains cells in a suspended, unattached state
Prevents stem cells from attachment-mediated differentiation
Prevents anchorage-dependent cells from dividing
Reduces binding of attachment and serum proteins to the substrate

Chambers are sterilized by gamma radiation and certified nonpyrogenic. Manufactured under GMP conditions and 100% leak-tested prior to shipping.