The NEXTFLEX® Rapid directional RNA-Sequence kit 2.0 is designed to prepare directional, strand specific RNA libraries for sequencing using Illumina® sequencers.

• High coverage uniformity with low duplication rate
• Optimized for use with 5 ng to 5 µg total RNA with reverse transcriptase and cleanup/size selection beads included
• Simple protocol validated with NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut™ rRNA depletion kit (human, mouse, rat)
• Designed to work with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index barcodes that allow a wide range of multiplexing (2 up to 1,526 samples in one run)
• Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations

The NEXTFLEX® Rapid directional RNA-seq kit 2.0 includes reverse transcriptase, necessary library preparation reagents, and cleanup/size selection beads optimized to ensure reliable performance. The kit involves a simple library preparation protocol that has been tested with the NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut™ rRNA depletion kit (human, mouse, rat) to accommodate total RNA as input. The NEXTFLEX® rapid directional RNA-seq kit 2.0 is designed to be used with NEXTFLEX® RNA-Seq 2.0 Unique dual index barcodes (6.25 μm), which are color-balanced and have undergone proprietary purity QC metrics to generate reliable sequencing results for every sample.