To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.
Specifications
- Description:EnzyFluo™ Inosine assay kit
- Size:100 tests
- Cat. no.:77441-688
Specifications
About this item
For determination of inosine concentration in cell lysate, serum, and other biological samples.
- Sensitive and accurate
- Use as little as 20 μl of sample
- Linear detection range in 96-well plate for 30 min incubation with 1 to 25 μM
- Simple and convenient
- The procedure involves addition of a single working reagent and incubation for 30 min at room temperature
- Fast and high-throughput
- Assays using 96-well plates and liquid handling system could allow simultaneous processing tens of thousands of samples per day
INOSINE is a purine nucleoside formed from Ribose and Hypoxanthine that can be found in most animal tissues and fluids. Inosine is most often found in tRNAs and is important for proper translation of the genetic code. It may play a role in cancer immunometabolism. It is degraded via the purine degradation pathway, first by purine nucleoside phosphorylase to hypoxanthine, then to xanthine by xanthine oxidase and then finally to uric acid by further action of xanthine oxidase.
Simple, direct and high-throughput assays for measuring inosine find wide applications in research and drug discovery. BioAssay Systems’ inosine assay kit uses a single working reagent that combines the enzyme reactions and color reaction in one step. The change in fluorescence intensity at λex/em = 530/585 nm is directly proportional to inosine concentration in the sample.