About this item
The PureCube Ni-INDIGO column/cartridge is an IMAC chromatography column prepacked with PureCube 100 INDIGO-Ni Agarose resin beads.
- Usage: Specific binding and purification of 6× His tagged proteins
- Specificity: Affinity to His-tagged proteins
- Binding capacity: >100 mg/ml
- Bead Ligand: Ni-INDIGO
- Bead size: 100 μm
- Filling quantity: Delivered as a 50% suspension
- pH stability: 2 to 14
- Chelator stability (e.g. EDTA): 20 mM
- Reducing agent stability (e.g. DTT): 20 mM
- Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Features - PureCube mini batch spin column:
Body material: Polypropylene
Filter pore size: 0.1 to 0.2 µm low binding PVDF
Max. volume: 20 ml
Max. g force: 1500 to 2000×g (swing-bucket rotor)
Min. g force: 100 to 200×g for 1 to 10 min
You can choose between two sizes, 1 ml bed volume and 5 ml bed volume. The PureCube cartridge/column that is used here is compatible with almost all commonly used FPLC systems like ÄKTA. We further recommend checking out our other Ni-INDIGO products.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. VWR will not lift restrictions for residential shipping addresses.