About this item
PureCube Ni-INDIGO magnetic beads/MagBeads were developed by Cube Biotech for His-tag protein purification.
- Usage: Specific binding and purification of 6× His tagged proteins
- Specificity: Affinity to His-tagged proteins
- Binding capacity: >100 mg/ml
- Bead ligand: Ni-INDIGO
- Bead size: 100 μm
- Filling quantity: Delivered as a 50% suspension
- pH stability: 2 to 14
- Chelator stability (e.g. EDTA): 20 mM
- Reducing agent stability (e.g. DTT): 20 mM
- Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Feature - PureCube mini batch spin column:
Body material: Polypropylene
Filter pore size: 0.1 to 0.2 µm low binding PVDF
Max. volume: 600 µl
Max. g force: 12000 to 14000×g (45-degree fixed angle rotor)
Min. g force: 2500×g for 1 min
Developed with the aim in mind of creating magnetic beads of the highest quality that can tolerate the highest concentrations of chelators like EDTA and DTT, which are often used in mammalian cell culture buffers. The resulting INDIGO ligand has EDTA and DTT stabilities of 20 mM each. That is highly superior to other competing magnetic beads like Ni-NTA.
In biotechnological and pharmaceutical processes in general, the INDIGO-Ni ligand has the advantage over the NTA ligand that no nickel leakage occurs due to its strong binding.
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