About this item
Our PureCube Cu-NTA agarose resins are small agarose resin beads with a diameter of 40 µm.
- Usage: Specific binding and purification of 6× His-tagged proteins, Binding of Copper-binding proteins
- Specificity: Affinity to His-tagged proteins, affinity to Copper-binding proteins
- Binding capacity: >80 mg/ml
- Bead Ligand: Cu-NTA
- Bead size: 40 μm
- Filling quantity: Delivered as a 50% suspension
- Metal ion capacity: >15 µeqv Cu²⁺/ml
- pH stability: 2 to 14
- Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
- Required equipment:
- Lysis Buffer
- Wash Buffer
- Elution Buffer
- Ice bath
- Refrigerated centrifuge for 50 ml tube (min 10000×g)
- 50 ml centrifuge tube
- Micropipettor and Micropipetting tips
- Disposable gravity flow columns with capped bottom outlet, 2 ml
- pH meter
- End-over-end shaker
- SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
PureCube Cu-NTA agarose resins are used for the purification of active His-tagged proteins from cells and the enrichment of Copper-binding proteins. Our year-long experience in manufacturing agarose resin led to the high yield of 80 mg protein per ml resin, which is leading in the market compared to other Cu-NTA suppliers. PureCube Cu-NTA resins are suited for batch spin columns and FPLC. Their small diameter provides them with great mechanical stability.
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- • Any other ID issued by the State that includes the business name & address
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