Stem cells possess the unique ability to differentiate into multiple lineages of cells including adipocytes. Lonza’s hMSC Adipogenic Differentiation BulletKit® Medium is a serum containing media system designed to induce adipogenic differentiation of bone marrow derived hMSC into mature, functionally active adipocytes in as few as 19 days.

Differentiation can then be validated by a variety of methods including the use of Lonza’s AdipoRed® Adipogenic Assay Reagent which provides a quantitative value related to the amount of adipogenic differentiation.

Partial List of Cells Differentiated with hMSC Adipogenic Differentiation BulletKit® Medium*:
Human bone marrow derived stem cells
C57BL/6 mice bone marrow derived stem cells
human adipose derived stem cells
Human periodontal ligament derived stem cells
Human hamstring tendon derived stem cells

The hMSC Adipogenic Differentiation BulletKit® Medium provides both an induction medium and a maintenance medium guaranteed to induce adipogenic differentiation of bone marrow derived hMSC into mature, functionally active adipocytes; Each bottle of the hMSC Adipogenic Differentiation BulletKit® Medium contains enough induction medium and maintenance medium to fully differentiate approximately 3 million bone marrow derived hMSC [approximately 15 wells in a 6-well plate format] into mature, functionally active adipocytes.

The hMSC Adipogenic Differentiation Medium is offered as a BulletKit® Medium (catalog no. PT-3004) which includes both the basal media for induction and maintenance and the necessary supplements for both adipogenic induction and maintenance of bone marrow derived hMSCs.

Lonza guarantees the performance of cells only if appropriate media and reagents are used exclusively and the recommended storage and use protocols are followed.