Neonatal, ventricular Rat Cardiomyocytes (P1-3) are high quality primary myocyte cells prepared by standardized methods, and are ready for immediate culture upon thaw. One vial of cardiomyocyte cells contains ≥4 million viable cells at ≥85% purity.

• Ready-To-Use
• No tissue dissection or animal handling required
• Increased data reproducibility
• Characteristic morphology and staining tests confirm tissue origin and cell type
• Negative for mycoplasma, bacteria, yeast and fungi

This will seed into 10 or more wells of a 24-well plate and 26 or more wells of a 96-well plate using the recommended plating densities and medium. When thawed and cultured you will obtain high viability, with excellent morphology and connectivity, and cells will display beating at 24 hours in culture.

Each lot of these cells test positive for functional syncytium formation, and stain positive for actinin. Cells also test negative for mycoplasma and bacteria. Primary cardiac myocyte cells need an appropriate substrate to adhere and survive. The preferred substrate is nitrocellulose.

Recommended medium: RCGM® BulletKit® (CC-4515)*
*RCGM® Rat Cardiomyocyte Growth Medium is specifically formulated for the growth and survival of rat neonatal cardiomyocytes in culture. Lonza offers customers a fully validated and optimized cardiomyocyte medium and growth factors to complement our Clonetics® Rat Cardiomyocytes, R-CM-561, as a complete system to study cardiac biology/toxicology.

Applications: Cardiovascular diseases, Hypertension, Inflammation, Cell contraction, Cardiac cell physiology, Ion channel flux, Cardiac lipid, metabolism and lipotoxicity, Cardiac ischemia and injury, Cardiac apoptosis, Cardiac cytotoxicity, Tissue engineering.