Nucleofector® Primary Cell Optimization 4D-Y Kit

Electroporation-based methods have so far required cells to be in suspension for transfection. However, the 4D-Nucleofector® Y Unit allows direct Nucleofection of cells in adherence. Cells which typically grow in adherence in culture can be kept and transfected by Nucleofection in their physiological state.

• Convenient and rapid determination of optimal conditions for Nucleofection of cells in adherence within one experiment

The primary cell optimization 4D-Nucleofector® Y kit is suited for rapid determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® Y Unit. Using one 24-well Dipping Electrode Array and our pmaxGFP® Vector as positive control, our two Primary Cell Nucleofector® Solutions for adherent Nucleofection (AD1 and AD2) are tested in combination with a pre-selected set of programs plus controls. The remaining 24-well Dipping Electrode Array can be used for optional fine tuning or performing the first experiment with the selected conditions.

The cells are cultured in the 24-well culture plates provided with the kit*. For the transfection step, a 24-well Dipping Electrode Array is inserted after replacing culture medium by the specific Nucleofector® Solution mixed with the DNA or RNA substrate.

Applications:
Determination of nucleofection conditions for primary cell types lacking an optimized protocol for the 4D-Nucleofector® Y unit.

Delivery information: Content:
2×4.5 ml AD1 4D-Nucleofector® Y solution.
2×4.5 ml AD2 4D-Nucleofector® Y solution.
4×1 ml Supplement 1.
6×150 μg pmaxGFP® vector (1 μg/μl in 10 mM Tris pH 8.0).
2×24-well Dipping Electrode Array.
2×Nunclon® Δ Surface 24-well plate (Nunc).