Electroporation-based methods have so far required cells to be in suspension for transfection. However, the 4D-Nucleofector® Y Unit allows direct Nucleofection of cells in adherence. Cells which typically grow in adherence in cell culture, can be kept and transfected by Nucleofection in their physiological state.

• Nucleofection of cells in adherence without the need for transfer in suspension
• Transfection efficiencies up to 70% combined with high viabilities

Applications:
Electroporation of cells in adherence.
For transfection of 2.5×10⁴ to 1×10⁵ cells.

The AD2 Primary Cell 4D-Nucleofector® Y Kit is one of our two kits that are suited for electroporation of primary cells in adherence when working with the 4D-Nucleofector® Y Unit. The cells are cultured in the 24-well culture plates provided with the kit* and for the transfection step a 24-well Dipping Electrode Array is inserted after replacing culture medium by the specific Nucleofector® Solution mixed with the DNA or RNA substrate.

This kit has been determined to be optimal for transfecting following cell types:
C2C12
Fibroblast, cardiac (rat)
Fibroblast, dermal, neonatal (human; NHDF-neo)
MEF (mouse)