Specifications
- Description:Primary cell optimization 96-well Nucleofector® kit
- Cat. no.:CA10064-144
- No. of Reactions:96
- Supplier No.:V4SP-9096
Specifications
About this item
The Primary cell optimization Nucleofector® kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an optimized protocol for the 4D-Nucleofector® 96-well unit.
- Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
- Optimal Nucleofection conditions determined on the 4D-Nucleofector® 96-well Unit are transferable to the 4D-Nucleofector® System or the 384-well HT Nucleofector® System
With the unique capability of the 4D-Nucleofector® 96-well Unit to address individual wells of a 96-well Nucleocuvette® Plate with different programs, primary cell optimizations are easily performed within one experiment.
Complete Optimization: Using one 96-well Nucleocuvette® Plate and our pmaxGFP® Vector as positive control, our five Primary Cell Nucleofector® Solutions P1 to P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 32 wells of the second 96-well Nucleocuvette® Plate can be used for optional fine tuning or performing the first experiment with the selected conditions.
Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector® Solutions and Nucleofector® Programs.
Basic Protocols are available for following cell groups:
Endothelial cells (mammalian)
Epithelial cells (mammalian)
Fibroblasts (mammalian)
Glial cells (mammalian)
Neurons (mammalian)
Smooth muscle cells (mammalian)
Stem cells (human)
Applications:
Determination of Nucleofection conditions for primary cell types lacking an optimized protocol for the 4D-Nucleofector® 96-well unit.
0.675 ml Nucleofector® Solution P1.
0.675 ml Nucleofector® Solution P2.
0.675 ml Nucleofector® Solution P3.
0.675 ml Nucleofector® Solution P4.
0.675 ml Nucleofector® Solution P5.
4×0.15 ml Supplement 1.
1×0.15 ml Supplement 3.
2×50 µg pmaxGFP® Vector (1 μg/μl in 10 mM Tris pH 8.0).
2×96-well Nucleocuvette® plates.
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