Nucleofector® Cell Line Optimization 4D-X Kit

The cell line optimization 4D-Nucleofector® X kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an optimized protocol for the 4D-Nucleofector® X unit.

• Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
• Optimal Nucleofection conditions determined in the 16-well Nucleocuvette® Strips are transferable to the 100 μl single Nucleocuvette® in the 4D-Nucleofector® X Unit
• Conditions are transferable between 4D Nucleofector® System, 96-well Shuttle® System and 384-well Nucleofector® System

With the unique capability of the 4D-Nucleofector® X Unit to address individual wells of a 16-well Nucleocuvette® Strip with different programs, cell line optimizations are easily performed within one experiment. Using three 16-well Nucleocuvette® Strips and our pmaxGFP® Vector as positive control, our three Cell Line Nucleofector® Solutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls. The remaining 16-well Nucleocuvette® Strip can be used for optional fine tuning or performing the first experiment with the selected conditions.

Applications: Determination of nucleofection conditions for cell lines lacking an optimized protocol for the 4D-Nucleofector® X unit.

Delivery information: Content:
0.675 ml Nucleofector® Solution SE.
0.675 ml Nucleofector® Solution SF.
0.675 ml Nucleofector® Solution SG.
3×0.15 ml Supplement 1.
50 µg pmaxGFP® Vector (1 μg/μl in 10 mM Tris pH 8.0).
4×20 µl 16-well Nucleocuvette® strips.